Mutations that happen after conception play an important role in autism, a study of nearly 6,000 families has found.
Over the past decade, mutations to more than 60 different genes have been linked with autism spectrum disorder (ASD), including spontaneous, non-inherited (de novo) mutations. But much of autism still remains unexplained.
The study, led by first author Elaine Lim, PhD, of Boston Children’s, and senior author Christopher Walsh, MD, PhD, of Boston Children’s and the Broad Institute, delved into an emerging category of de novo mutations: those found in only a subset of our cells.
De novo mutations can occur in a parent’s sperm or egg, or they can occur after fertilization, arising in an embryonic cell. These are known as post-zygotic mutations or PZMs (also known as somatic mutations). The later PZMs occur during embryonic development, the fewer cells will carry them, making them harder to detect.
“If the mutation is in a very small fraction of all cells, it will be missed by whole-exome sequencing,”
Three Genetic Sequencing Technologies
To locate post-zygotic mutations, Lim, Walsh and colleagues obtained whole-exome sequencing data previously gathered from 5,947 families, courtesy of the Simons Foundation Autism Research Initiative (SFARI) Simplex Collection, the Autism Sequencing Consortium and Autism Speaks. They then re-sequenced some of the DNA from these children using three independent sequencing technologies in parallel.
Based on their findings, they classified 7.5 percent of ASD subjects’ de novo mutations as PZMs. Of these, 83 percent had not been picked up in the original analysis of their genome sequence.
Some PZMs affected genes already known to be linked to autism or other neurodevelopmental disorders (such as SCN2A, HNRNPU and SMARCA4), but sometimes affected these genes in different ways. Many others were in genes known to be active in brain development (such as KLF16 and MSANTD2) but not previously associated with ASD.
The connection of these genes to autism may have been missed before because the earlier studies focused on mutations that knocked down gene function.
“Some of the postzygotic mutations we found represented a gain of function, not a loss of function,”
says Lim, who is also affiliated with the Wyss Institute for Biologically Inspired Engineering.
Amygdala And Autism
To estimate the developmental timing of the mutations and the brain regions affected, Lim, Walsh and colleagues compared their sequencing data, which mostly came from blood DNA samples, with publicly available gene expression data from brain autopsies representing different ages (prenatal through adult) via the BrainSpan Project.
“By overlapping the data, we can start to map where in the brain these genes are expressed and when the mutations occurred during development,” says Lim.
These analyses showed that PZMs in the subjects with ASD occur disproportionately in genes expressed in the amygdala.
“This was exciting to us, in that the amygdala has been proposed as an important region of the brain in autism,” says Lim.
Overall, the work adds to evidence that complex brain disorders, such as epilepsy, intellectual disability, schizophrenia and brain malformations, can arise from non-inherited mutations that occur at some point during prenatal development.
Elaine T Lim, Mohammed Uddin, Silvia De Rubeis, Yingleong Chan, Anne S Kamumbu, Xiaochang Zhang, Alissa M D’Gama, Sonia N Kim, Robert Sean Hill, Arthur P Goldberg, Christopher Poultney, Nancy J Minshew, Itaru Kushima, Branko Aleksic, Norio Ozaki, Mara Parellada, Celso Arango, Maria J Penzol, Angel Carracedo, Alexander Kolevzon, Christina M Hultman, Lauren A Weiss, Menachem Fromer, Andreas G Chiocchetti, Christine M Freitag, Autism Sequencing Consortium, George M Church, Stephen W Scherer, Joseph D Buxbaum & Christopher A Walsh Rates, distribution and implications of postzygotic mosaic mutations in autism spectrum disorder Nature Neuroscience (2017) doi:10.1038/nn.4598